Information for Physicians
Indications for H3K27M mutation testing
- Liquid biopsy confirmation of radiographic diagnosis of diffuse midline glioma, H3K27M variant
- Tissue biopsy confirmation of H3K27M mutation when immunohistochemistry not definitive
- Liquid biopsy confirmation of H3K27M mutation for clinical trial eligibility
- Serial liquid biopsy testing for H3K27M as therapeutic response biomarker in context of clinical trial
ResourcePath ddPCR assays for H3K27M mutations
- H3F3A c.83A>T; K27M (sometimes expressed as p.K28M) samples being accepted during analytic validation
- HIST1H3B c.83A>T; K27M (sometimes expressed as p.K28M) samples being accepted during analytic validation
- H3F3A c.103G>T;p.G35W anticipated late summer 2019
- H3F3A c.103G>A;p.G35R anticipated late summer 2019
Sample types accepted for H3F3A and HIST1H3B ddPCR testing and processing requirements
Specimen Type | Collection Container | Processing | Minimum Volume |
Cerebrospinal Fluid (CSF) | CSF collection kit | Required at collection. See instructions below | 1 mL for ddPCR 2-5 mL for NGS |
Plasma | Purple Top/EDTA Tube | Required at collection. See instructions below | 1 mL minimum |
Whole Blood | Streck Cell Free DNA BCT Tube | None | Fill tube completely (~ 8 mL) |
FFPE biopsy tissue | Paraffin block or scrolls | None | Two 10-micron scrolls + 1 H&E or digital image of H&E to assess tumor content |
- For each patient, please complete and send back the ResourcePath Molecular Test Requisition
- All materials must be properly packaged and labeled to indicate the general nature of the materials being transported. The laboratory only accepts specimens considered Class B/non-infectious, and should be shipped using a FedEx Clinical Pak or equivalent.
- The lab accepts specimens Monday through Friday. For samples collected on a Friday, please place them at the appropriate storage conditions and ship the following Monday using the standard overnight shipping option.
Please ship all specimens to:
Resourcepath, LLC
45945 Trefoil Lane. Suite 175
Sterling, VA 20166, USA
info@resourcepath.net
(571) 375-0755
Please send an email to info@resourcepath.net when the specimens have shipped and include the following information:
- Date shipped
- Carrier
- Tracking number
- Number of specimens