Information for Physicians

Indications for H3K27M mutation testing

  • Liquid biopsy confirmation of radiographic diagnosis of diffuse midline glioma, H3K27M variant
  • Tissue biopsy confirmation of H3K27M mutation when immunohistochemistry not definitive
  • Liquid biopsy confirmation of H3K27M mutation for clinical trial eligibility
  • Serial liquid biopsy testing for H3K27M as therapeutic response biomarker in context of clinical trial

ResourcePath ddPCR assays for H3K27M mutations

  • H3F3A c.83A>T; K27M (sometimes expressed as p.K28M) samples being accepted during analytic validation 
  • HIST1H3B c.83A>T; K27M (sometimes expressed as p.K28M) samples being accepted during analytic validation 
  • H3F3A c.103G>T;p.G35W anticipated late summer 2019
  • H3F3A c.103G>A;p.G35R anticipated late summer 2019

 

Sample types accepted for H3F3A and HIST1H3B ddPCR testing and processing requirements

Specimen Type

Collection Container

Processing

Minimum Volume

 Cerebrospinal Fluid (CSF)

CSF collection kit

Required at collection. See instructions below

1 mL for ddPCR

2-5 mL for NGS

 Plasma

Purple Top/EDTA Tube

Required at collection. See instructions below

1 mL minimum

 Whole Blood

Streck Cell Free DNA BCT Tube 

None

Fill tube completely (~ 8 mL)

FFPE biopsy tissue

Paraffin block or scrolls

None

Two 10-micron scrolls + 1 H&E or digital image of H&E to assess tumor content

  • For each patient, please complete and send back the ResourcePath Molecular Test Requisition
  • All materials must be properly packaged and labeled to indicate the general nature of the materials being transported. The laboratory only accepts specimens considered Class B/non-infectious, and should be shipped using a FedEx Clinical Pak or equivalent.
  • The lab accepts specimens Monday through Friday. For samples collected on a Friday, please place them at the appropriate storage conditions and ship the following Monday using the standard overnight shipping option.

Please ship all specimens to:

Resourcepath, LLC
45945 Trefoil Lane. Suite 175
Sterling, VA 20166, USA


(571) 375-0755

Please send an email to info@resourcepath.net when the specimens have shipped and include the following information:

  • Date shipped
  • Carrier
  • Tracking number
  • Number of specimens